PCR detection of Gene Stmpr1, Stmpr2 and Stmpr3 encoding protease of Stenotrophomonas malltophilia isolated from different infection

Stenotrophomonas maltophilia is an emerging opportunistic nosocomial pathogen, with many virulence factor and causing various infections. Therefore this study conducted to characterization molecular investigation of some extracellular particularly serin protease detection gene encoding (Stmpr1, Stmpr2 Stmpr3 genes) in order determinants the role bacteria infection Al- Najaf province. 485 clinical specimens were collected from different 315 (64.9%) male 170(35.1%) female, cultured on suitable media cultivated at 37 C, The results showed that 373(76.9%) gave bacterial growth while 112(23.1%) appeared no after identification isolate, 20 isolate identified recovered infections, those 12 isolates specimens, 8 PCR used determinant predominant genes among Stenotrophomona isolates, revealed all (100%) carried stmpr1, stmpr2 (85%) genes, which positive bands correct product size.